yap sirna  (Sangon Biotech)

Journal: bioRxiv

Article Title: Modeling Meibomian Gland Development and Dysfunction: A Mouse-Derived Organoid System Reveals Hippo-YAP as a Critical Regulator

doi: 10.64898/2026.05.13.724874

Figure Lengend Snippet: (A and B) Bright-field images of mMGOs treated with verteporfin, with DMSO as control. The axial length and the surface area of mMGOs were measured using ImageJ (n=35). (C and D) Immunofluorescence of Ki67 and EdU staining in mMGOs, nuclei were counterstained with DAPI. (E and F) Percentage of Ki67 and EdU positive cells in (C and D) (n=8). (G) Relative mRNA expression of Ki67 (n=4) and Pcna (n=3) in mMGOs. (H and I) Western blot analysis of YAP in mMGOs transfected with 3 types of YAP-siRNA, with NC-siRNA as control, the protein levels were quantified by densitometry (n=3). (J and K) Bright-field images of mMGOs transfected with YAP-siRNA 3, with NC-siRNA as control. And the axial length and the surface area of mMGOs were quantified (n=24). (L and M) Immunofluorescence of Ki67 and EdU staining in mMGOs, nuclei were counterstained with DAPI. (N and O) Percentage of Ki67 and EdU positive cells in (L and M) (n=4). Scale bars represent 100 µm in (A) and (J), 50 µm in (C), (D), (L) and (M).

Article Snippet: YAP expression was knocked down in mMGOs transfection with the YAP siRNA (Sangon Biotech) using RNATransMate (Sangon Biotech, E607402) following the manufacturer’s instruction, with negative control (NC) siRNA as control.

Techniques: Control, Immunofluorescence, Staining, Expressing, Western Blot, Transfection

Journal: bioRxiv

Article Title: Modeling Meibomian Gland Development and Dysfunction: A Mouse-Derived Organoid System Reveals Hippo-YAP as a Critical Regulator

doi: 10.64898/2026.05.13.724874

Figure Lengend Snippet: (A and B) Immunofluorescence of YAP in MG of 8-week-old and 24-month-old mice, nuclei were counterstained with DAPI. And the ratio of nuclear YAP to cytoplasm YAP, three cells were randomly selected for measurement and averaged in each sample (n=5). (C and D) Bright-field images of mMGOs treated with LPS, with PBS as control. Number of budding (n=62) and the surface area (n=57) of mMGOs were quantified. (E) Immunofluorescence of Ki67 in mMGOs, nuclei were counterstained with DAPI. (F) TUNEL staining of mMGOs, nuclei were counterstained with DAPI. (G) Immunofluorescence of K10 in mMGOs, nuclei were counterstained with DAPI. (H) Percentage of Ki67 positive cells in (C) (n=6). (I) The percentage of TUNEL positive cells in (D) (n=5). (J) The intensities of K10 in (E) (n=3). (K and L) Immunofluorescence of YAP in LPS-induced MGD model in vitro , nuclei were counterstained with DAPI. And the ratio of nuclear YAP to cytoplasm YAP (n=4). (M and N) Bright-field images of mMGOs transfected with YAP-siRNA 3 after maturation, with NC-siRNA as control. Changes of budding number were quantified (n=3). (O and P) Bright-field images of mMGOs cultured with XMU-MP-1 after maturation, with DMSO as control. Number of budding (n=33) and the surface area (n=39) of mMGOs were quantified. Scale bars represent 250 µm in (C), 100 µm in (M) and (O), 50 µm in (E), (F) and (G), 25 µm in (A) and (K).

Article Snippet: YAP expression was knocked down in mMGOs transfection with the YAP siRNA (Sangon Biotech) using RNATransMate (Sangon Biotech, E607402) following the manufacturer’s instruction, with negative control (NC) siRNA as control.

Techniques: Immunofluorescence, Control, TUNEL Assay, Staining, In Vitro, Transfection, Cell Culture

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Vitexin Attenuates the Growth and Glycolysis of Acute Myeloid Leukemia Cells by Suppressing the HIF ‐1α‐Modulated YAP Pathway Under Hypoxic Conditions

doi: 10.1002/kjm2.70111

Figure Lengend Snippet: The YAP pathway is regulated by HIF‐1α in hypoxia‐exposed HL‐60 and KG‐1α cells. (A, B) HIF‐1α protein level was decreased in HL‐60 and KG‐1α cells 48 h after transfection with siHIF‐1α. *** p < 0.001 vs. siCtrl group. (C, D) HIF‐1α protein level was increased in HL‐60 and KG‐1α cells 48 h after transfection with HIF‐1α overexpression vector. *** p < 0.001 vs. Vector group. (E‐G) HL‐60 and KG‐1α cells were transfected with siCtrl or siHIF‐1α under normoxic or hypoxic conditions for 48 h. Western blot analysis was conducted to detect the expression levels of p‐YAP and YAP. *** p < 0.001 vs. Normoxia + siCtrl group. ## p < 0.01, ### p < 0.001 vs. Hypoxia + siCtrl group. (H‐J) HL‐60 and KG‐1α cells were transfected with control empty vector or HIF‐1α overexpression vector under normoxic or hypoxic conditions for 48 h. Western blot analysis was conducted to detect the expression levels of p‐YAP and YAP. Data are presented as mean ± SD. ** p < 0.01, *** p < 0.001 vs. Normoxia + Vector group. # p < 0.05, ## p < 0.01 vs. Hypoxia + Vector group. siCtrl, control siRNA. siHIF‐1α, siRNA targeting HIF‐1α. Vector, control empty vector. HIF‐1α, HIF‐1α overexpression vector.

Article Snippet: For constructing HIF‐1α‐ or YAP‐depleting cells, 10 μL of HIF‐1α siRNA (10 μM, siHIF‐1α), or YAP siRNA (10 μM, siYAP), or control siRNA (10 μM, siCtrl) obtained from Santa Cruz (Santa Cruz, CA) were transfected into HL‐60 and KG‐1α cells using a Lipofectamine RNAiMAX transfection reagent (Thermo Fisher).

Techniques: Transfection, Over Expression, Plasmid Preparation, Western Blot, Expressing, Control

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Vitexin Attenuates the Growth and Glycolysis of Acute Myeloid Leukemia Cells by Suppressing the HIF ‐1α‐Modulated YAP Pathway Under Hypoxic Conditions

doi: 10.1002/kjm2.70111

Figure Lengend Snippet: Roles of HIF‐1α or the YAP pathway in the inhibitory effect of vitexin on hypoxia‐induced AML cell growth. (A) YAP was successfully knocked down in HL‐60 and KG‐1α cells through transfection with siYAP. Data are presented as mean ± SD. *** p < 0.001 vs. siCtrl group. HL‐60 and KG‐1α cells co‐transfected with siCtrl/siYAP and Vector/HIF‐1α were treated with or without 40 μM vitexin under normoxic or hypoxic conditions for 48 h. (B, C) MTT assay was performed to assess cell viability. Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001. (D‐F) EdU incorporation assay was conducted to assess cell proliferation. Scale bar = 20 μm. Data are presented as mean ± SEM. *** p < 0.001. (G, H) Western blot analysis was conducted to detect the expression levels of PCNA and Ki67. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. siCtrl, control siRNA. siYAP, siRNA targeting YAP. Vector, control empty vector. HIF‐1α, HIF‐1α overexpression vector.

Article Snippet: For constructing HIF‐1α‐ or YAP‐depleting cells, 10 μL of HIF‐1α siRNA (10 μM, siHIF‐1α), or YAP siRNA (10 μM, siYAP), or control siRNA (10 μM, siCtrl) obtained from Santa Cruz (Santa Cruz, CA) were transfected into HL‐60 and KG‐1α cells using a Lipofectamine RNAiMAX transfection reagent (Thermo Fisher).

Techniques: Transfection, Plasmid Preparation, MTT Assay, Western Blot, Expressing, Control, Over Expression

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: Vitexin Attenuates the Growth and Glycolysis of Acute Myeloid Leukemia Cells by Suppressing the HIF ‐1α‐Modulated YAP Pathway Under Hypoxic Conditions

doi: 10.1002/kjm2.70111

Figure Lengend Snippet: Roles of HIF‐1α or the YAP pathway in the inhibitory effect of vitexin on hypoxia‐induced glycolysis. HL‐60 and KG‐1α cells co‐transfected with siCtrl/siYAP and Vector/HIF‐1α were treated with or without 40 μM vitexin under normoxic or hypoxic conditions for 48 h. (A, B) Measurement of glucose uptake. Data are presented as mean ± SEM. (C, D) Measurement of lactate production. Data are presented as mean ± SEM. (E‐J) qRT‐PCR for detecting the mRNA expression levels of HK2 (E, F), PKM2 (G, H), and LDHA (I, J). Data are presented as mean ± SD. (K‐R) Western blot analysis for detecting the protein expression levels of HK2, PKM2, and LDHA in HL‐60 (K‐N) and KG‐1α (O‐R) cells. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. siCtrl, control siRNA. siYAP, siRNA targeting YAP. Vector, control empty vector. HIF‐1α, HIF‐1α overexpression vector.

Article Snippet: For constructing HIF‐1α‐ or YAP‐depleting cells, 10 μL of HIF‐1α siRNA (10 μM, siHIF‐1α), or YAP siRNA (10 μM, siYAP), or control siRNA (10 μM, siCtrl) obtained from Santa Cruz (Santa Cruz, CA) were transfected into HL‐60 and KG‐1α cells using a Lipofectamine RNAiMAX transfection reagent (Thermo Fisher).

Techniques: Transfection, Plasmid Preparation, Quantitative RT-PCR, Expressing, Western Blot, Control, Over Expression